Protein Analysis and Nanoscale Protein Detection

NanoInk's Nano BioDiscovery Division provides a new generation of array-based instrument systems, assay kits, and contract services for nanoscale protein detection. Our platforms combine patented Dip Pen Nanolithography® (DPN®) nanofabrication technology with optimized substrates and ultra-sensitive protein detection to miniaturize and multiplex protein analysis. As the only true nanoscale protein analysis company, we help our customers address major proteomic challenges.

Traditional Protein Array Technology

Traditional protein array technologies that use pin spotting often print inconsistent features, leading to poor sensitivity and reproducibility. The Ink jet printing process is known to aerosolize the reagents during the protein array printing process which can cause splattering, higher localized backgrounds and lower assay signal to noise ratios. Other protein detection techniques often require large amounts of sample material and reagents and exhibit prohibitively slow reaction kinetics.

Superior DPN Nanoarrays

By contrast, DPN nanoarray patterns exhibit highly uniform and repeatable features within and between nanoarrays. This consistency enables improved sensitivity, increased reproducibility and low non-specific binding. As a result, even low-abundance protein studies and analysis are possible. DPN’s ability to automate protein sub-array deposition over millimeter areas with nanometer precision and then fluorescently image these nanoarrays with 0.5 µm resolution translates to multiplexed, high throughput protein analysis on a single slide. And since nanoarray deposition consumes only femtoscale quantities of proteins and reagents, it enables rapid reaction kinetics and assay cost-savings with only 2 µl of sample. NanoInk’s nanoscale systems enable scientists to probe proteins buried deep in the proteome for more complete protein analysis and protein detection.

For more information, call us at 1-847-679-3432, and ask for Nano BioDiscovery.

Think big. Work nanoscale.

NanoArray Assays: More Proteomic Data From Less Sample.
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Schematic of a human HER2 receptor and EGF receptor kinase nanoarray assay